Issue 26, 2021
From the journal:

Chemical Communications

Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis†

Chikara Dohno, ORCID logo *a   Masaki Hagihara, ORCID logo *b   Nursakinah Binti Mohd Zaifuddin,a   Mizuki Nihei,b   Kaoru Saitob  and  Kazuhiko Nakatani ORCID logo *a  

* Corresponding authors

a The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
E-mail: cdohno@sanken.osaka-u.ac.jp, nakatani@sanken.osaka-u.ac.jp

b Graduate School of Science and Technology, Hirosaki University, Hirosaki 036-8561, Japan
E-mail: hagihara@hirosaki-u.ac.jp

Abstract

We demonstrated that a synthetic ligand NA, which selectively binds to a 5′-CAG-3′/5′-CAG-3′ triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG)20 template gave shortened nascent strands mainly containing 3–6 CTG units in the presence of NA.

Graphical abstract: Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis

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Article information

DOI
https://doi.org/10.1039/D1CC00349F
Article type
Communication
Submitted
20 Jan 2021
Accepted
19 Feb 2021
First published
19 Feb 2021

Chem. Commun., 2021,57, 3235-3238

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Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis

C. Dohno, M. Hagihara, N. Binti Mohd Zaifuddin, M. Nihei, K. Saito and K. Nakatani, Chem. Commun., 2021, 57, 3235 DOI: 10.1039/D1CC00349F

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