Ionic liquid-assisted chemiluminescent immunoassay of prostate specific antigen using nanoceria as an alkaline phosphatase-like nanozyme label

Abstract

An alkaline phosphatase-like nanozyme was applied for an immunoassay for the first time. By using nanoceria as the alkaline phosphatase-like catalytic label and CDP-star as the substrate, the chemiluminescent detection of prostate specific antigen was demonstrated. More importantly, the addition of ionic liquid can significantly increase the sensitivity of the immunoassay. With the aid of ionic liquid, an order of magnitude improvement in the sensitivity was achieved with a detection limit of 53 fg mL⁻¹.