Issue 22, 2021
From the journal:

Chemical Communications

A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA†

Bingyao Wang,‡a   Yi Chen,‡a   Xiong Zhang,a   Zhuoran Jiang,a   Yafen Wang, ORCID logo a   Kun Chen, ORCID logo a   Fang Wang,b   Xiaocheng Weng ORCID logo *a  and  Xiang Zhou ORCID logo *a  

* Corresponding authors

a The Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, China
E-mail: xzhou@whu.edu.cn, xcweng@whu.edu.cn
Fax: +86-27-68756663
Tel: +86-27-68756663

b Wuhan University School of Pharmaceutical Sciences, Wuhan, China

Abstract

We report a new method for dU detection in genomic DNA combined with UNG excision and fluorescent probe labeling. UNG can remove uracil bases to introduce abasic sites, which can react with NRNO to produce intense fluorescence because of the inhibition of the PET effect. It can also cause the polymerase extension to stop to provide details of dU site information.

Graphical abstract: A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA

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Article information

DOI
https://doi.org/10.1039/D1CC00016K
Article type
Communication
Submitted
02 Jan 2021
Accepted
04 Feb 2021
First published
04 Feb 2021

Chem. Commun., 2021,57, 2784-2787

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A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA

B. Wang, Y. Chen, X. Zhang, Z. Jiang, Y. Wang, K. Chen, F. Wang, X. Weng and X. Zhou, Chem. Commun., 2021, 57, 2784 DOI: 10.1039/D1CC00016K

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