Issue 20, 2021

Precise identification of an RNA methyltransferase's substrate modification site

Abstract

Here we report a simple and nonradioactive biochemical assay which is capable of accurately determining the substrate methylation sites of human RNA N6-methyladenosine methyltransferases METTL3/METTL14 and METTL16. This method employs enzyme-assisted chemical labelling of a specific base in an RNA substrate with the assistance of an allyl-substituted methyltransferase cofactor, and enables precise identification of the labelling site by a mutation signal from standard nucleic acid sequencing. Our method provides a platform to investigate the enzymatic methylations of long and structurally complex RNA substrates, and facilitates the discovery of new methyltransferases.

Graphical abstract: Precise identification of an RNA methyltransferase's substrate modification site

Supplementary files

Article information

Article type
Communication
Submitted
20 Dec 2020
Accepted
28 Jan 2021
First published
29 Jan 2021

Chem. Commun., 2021,57, 2499-2502

Precise identification of an RNA methyltransferase's substrate modification site

S. Xiang, M. Gao, J. Cao, X. Shu, M. Cheng, F. Wang, T. Deng and J. Liu, Chem. Commun., 2021, 57, 2499 DOI: 10.1039/D0CC08260K

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