Issue 14, 2021
From the journal:

Chemical Communications

Genetically encoding ε-N-benzoyllysine in proteins†

Yanli Ji,a   Conghui Ren,a   Hui Miao,a   Zhili Pang,a   Ruotong Xiao,a   Xiaochen Yanga  and  Weimin Xuan ORCID logo *a  

* Corresponding authors

a State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China
E-mail: wxuan@nankai.edu.cn

Abstract

Lysine ε-N-benzoylation is a recently identified PTM occurring on histone proteins, and herein we genetically encoded ε-N-benzoyl-lysine (BzK) into recombinant proteins in E. coli and mammalian cells, and applied it for the modification of histone proteins and the analysis of sirtuin debenzoylase activity.

Graphical abstract: Genetically encoding ε-N-benzoyllysine in proteins

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Article information

DOI
https://doi.org/10.1039/D0CC07954E
Article type
Communication
Submitted
08 Dec 2020
Accepted
08 Jan 2021
First published
08 Jan 2021

Chem. Commun., 2021,57, 1798-1801

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Genetically encoding ε-N-benzoyllysine in proteins

Y. Ji, C. Ren, H. Miao, Z. Pang, R. Xiao, X. Yang and W. Xuan, Chem. Commun., 2021, 57, 1798 DOI: 10.1039/D0CC07954E

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