Issue 21, 2021

Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

Abstract

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.

Graphical abstract: Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

Supplementary files

Article information

Article type
Communication
Submitted
25 Nov 2020
Accepted
22 Jan 2021
First published
22 Jan 2021

Chem. Commun., 2021,57, 2661-2664

Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

J. Lee, K. J. Schwarz, H. Yu, A. Krüger, E. V. Anslyn, A. D. Ellington, J. S. Moore and M. C. Jewett, Chem. Commun., 2021, 57, 2661 DOI: 10.1039/D0CC07740B

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