Issue 3, 2021

Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings

Abstract

The immune response of bone implants is closely related to the interaction between macrophages and biomaterial surfaces. In this work, the polarization behavior of mouse bone marrow-derived macrophages (BMDMs), including their morphology and expression of phenotypic markers, genes and cytokines, on charged surfaces with different potential intensities was systematically explored. We found that the charged surface could effectively promote BMDM polarization, and a higher potential intensity was conducive to the upregulation of the polarization of BMDMs into the M2 phenotype. Based on the analysis of the signaling pathways involved in integrins (αMβ2 and α5β1) and the potassium ion channel (Kv1.3), a possible underlying mechanism revealed that the integrin originated signaling pathways could more dominantly regulate macrophage polarization to the M2 phenotype. The present work therefore demonstrates that the surface charge, as a physicochemical property of material surfaces, could effectively regulate macrophage polarizations, which may provide an immunoregulation view for the surface design of biomaterials.

Graphical abstract: Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings

Supplementary files

Article information

Article type
Paper
Submitted
19 Sep 2020
Accepted
12 Nov 2020
First published
14 Nov 2020

Biomater. Sci., 2021,9, 874-881

Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings

Z. Wang, X. He, B. Tang, X. Chen, L. Dong, K. Cheng and W. Weng, Biomater. Sci., 2021, 9, 874 DOI: 10.1039/D0BM01604G

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