The separation characteristics and performance evaluation of the silica-based poly(pentabromostyrene) stationary phase in capillary electrochromatography

Abstract

A mixed-mode capillary column packed with silica-based poly(pentabromostyrene) particles (denoted as SiO₂@pPBS) was prepared and applied to capillary electrochromatography (CEC) separation. With the presence of benzene rings and bromine atoms in polymer chains, the SiO₂@pPBS column provides a reversed-phase/hydrophilic mixed-mode retention mechanism owing to hydrophilic, hydrophobic and π–π interactions between the stationary phase and various analytes, including alkylbenzenes, polycyclic aromatic hydrocarbons, nucleosides, phenols and anilines. In CEC mode, the separation behavior of charged solutes is not only related to the interaction with the stationary phase, but also influenced by electrophoretic effects, which may lead to different selectivities compared to high performance liquid chromatography (HPLC). A column efficiency of up to 1.22 × 10⁵ N m⁻¹ was achieved for p-chloroaniline. Besides, the RSDs of retention time of anilines for run to run (n = 5), day to day (n = 5) and column to column (n = 3) were all less than 4.4%. Finally, the SiO₂@pPBS capillary column was applied to the separation of coking wastewater with satisfactory results. All the results demonstrated that the SiO₂@pPBS capillary packed column with RP/HILIC mixed-mode has great application potential.