Development of a bimetal–organic framework–polypyrrole composite as a novel fiber coating for direct immersion solid phase microextraction in situ supercritical fluid extraction coupled with gas chromatography for simultaneous determination of furfurals in dates

Abstract

A new, simple, hyphenated technique couples supercritical fluid extraction and direct immersion SPME with GC-FID (SFE-DI-SPME-GC-FID) for the determination of 2-furaldehyde (2-F) and 5-hydroxymethylfurfural (5-HMF) in solid foods. A bimetal-organic framework-polypyrrole composite was grown in situ on stainless steel wire in solution and used as a novel solid phase microextraction (SPME) fiber coating. A central composite design based on a 2ⁿ⁻¹ fractional factorial experimental design was employed to optimize the SFE conditions for 2-F and 5-HMF at a pressure of 325 atm, temperature of 35 °C, dynamic extraction time of 15 min, and modifier volume of 150 μL. Also, the factors related to the solid-phase microextraction method including ionic strength, desorption time and temperature together with extraction time and temperature were optimized prior to the gas chromatography analysis. Under the optimal conditions, the limits of detection were in the range of 1.28–5.92 μg kg⁻¹. This method showed good linearity for 2-F and 5-HMF in the ranges of 40–50 000 and 4540–500 000 μg kg⁻¹, respectively, with coefficients of determination more than 0.9995. Single fiber repeatability and fiber-to-fiber reproducibility were less than 6.76% and 9.12%, respectively. The new method was successfully utilized to determine the amounts of 2-F and 5-HMF in the real solid food matrix without the need for tedious pretreatments.