Issue 38, 2021

A novel method to detect hPG80 (human circulating progastrin) in the blood

Abstract

hPG80 (human circulating progastrin) is produced and released by cancer cells. We recently reported that hPG80 is detected in the blood of patients with cancers from different origins, suggesting its potential utility for cancer detection. To accurately measure hPG80 in the blood of patients, we developed the DxPG80 test, a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This test quantifies hPG80 in EDTA plasma samples. The analytical performances of the DxPG80 test were evaluated using standard procedures and guidelines specific to ELISA technology. We showed high specificity for hPG80 with no cross-reactivity with human glycine-extended gastrin (hG17-Gly), human carboxy-amidated gastrin (hG17-NH2) or the CTFP (C-Terminus Flanking Peptide) and no interference with various endogenous or exogenous compounds. The test is linear between 0 and 50 pM hPG80 (native or recombinant). We demonstrated a trueness of measurement, an accuracy and a variability of hPG80 quantification with the DxPG80 test below the 20% relative errors as recommended in the guidelines. The limit of detection of hPG80 and the limit of quantification were calculated as 1 pM and 3.3 pM respectively. In conclusion, these results show the strong analytical performance of the DxPG80 test to measure hPG80 in blood samples.

Graphical abstract: A novel method to detect hPG80 (human circulating progastrin) in the blood

Article information

Article type
Paper
Submitted
09 Jun 2021
Accepted
03 Sep 2021
First published
03 Sep 2021
This article is Open Access
Creative Commons BY-NC license

Anal. Methods, 2021,13, 4468-4477

A novel method to detect hPG80 (human circulating progastrin) in the blood

M. Cappellini, M. Flaceliere, V. Saywell, J. Soule, E. Blanc, F. Belouin, E. Ortiz, L. Canterel-Thouennon, S. Poupeau, S. Tigrett, B. Vire, P. Liaud, M. Blairvacq, D. Joubert and A. Prieur, Anal. Methods, 2021, 13, 4468 DOI: 10.1039/D1AY00986A

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