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Issue 21, 2021
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Multiresidue determination of 19 anabolic steroids in animal oil using enhanced matrix removal lipid cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry

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Abstract

A method for sensitive analysis of 19 anabolic steroids (AS) in animal oil using enhanced matrix removal lipid (EMR-Lipid) cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. Oil samples were extracted with 20 mL of acetonitrile aqueous solution and purified using EMR-Lipid cartridges. The eluent was evaporated to dryness under nitrogen and analyzed by UHPLC-MS/MS using 0.1% formic acid–acetonitrile and 0.1% formic acid–water solutions as the mobile phase via gradient elution. The method effectively removed unwanted matrix co-extractives better than other extraction cleanup techniques while still delivering acceptable recovery results for most of the AS. The established quantification method showed AS recovery in the range of 72.9–110.7% with good precision (relative standard deviation < 15%).

Graphical abstract: Multiresidue determination of 19 anabolic steroids in animal oil using enhanced matrix removal lipid cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry

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Article information


Submitted
15 Mar 2021
Accepted
14 Apr 2021
First published
15 Apr 2021

Anal. Methods, 2021,13, 2374-2383
Article type
Communication

Multiresidue determination of 19 anabolic steroids in animal oil using enhanced matrix removal lipid cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry

Y. Jiafeng, S. Decheng, L. Xiaoyong, L. Yang, L. Guangyu and B. S. Min, Anal. Methods, 2021, 13, 2374
DOI: 10.1039/D1AY00437A

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