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Issue 22, 2021
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A fluorescent PET probe based on polyethyleneimine-Ag nanoclusters as a reversible, stable and selective broad-range pH sensor

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Abstract

In this work, nanoclusters (NCs) of Cu and Ag capped with hyperbranched polyethyleneimine (PEI) were prepared using chemical reduction by a one-step hydrothermal method. The PEI coated-NCs were characterized by high-resolution transmission electron microscopy, ζ potential, thermogravimetric analysis, dynamic light scattering, Fourier-transform infrared, UV-visible, and fluorescence spectroscopy. The PEI-NCs exhibited strong absorption and fluorescence, high stability, and excellent water dispersibility. The resulting PEI-NCs showed a reversible and linear response of fluorescence intensity with pH over a wide range (3–11); however, PEI-AgNCs showed a better reversibility and sensitivity than PEI-CuNCs. Unlike several types of pH sensors based on modified NCs, which are based on a nanoparticle aggregation/disaggregation mechanism, the response of our sensor is based on a photoinduced electron transfer process, which gives it a high reversibility. This method was successfully applied in pH measurements in tap water and green tea samples, with excellent results, indicating its practical utility for these applications. A visual device was obtained by immobilizing PEI-AgNCs into agarose hydrogels at different pH values. The results show that the proposed sensor can be used as a pH visual detector. Besides, the light emission of the nanosensor was corroborated by fluorescence microscopy, confirming that the nanosensor based on PEI-AgNCs has great potential to be used in cellular imaging.

Graphical abstract: A fluorescent PET probe based on polyethyleneimine-Ag nanoclusters as a reversible, stable and selective broad-range pH sensor

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Supplementary files

Article information


Submitted
22 Feb 2021
Accepted
13 Apr 2021
First published
27 Apr 2021

Anal. Methods, 2021,13, 2495-2503
Article type
Paper

A fluorescent PET probe based on polyethyleneimine-Ag nanoclusters as a reversible, stable and selective broad-range pH sensor

A. P. Reyes-Cruzaley, A. Ochoa-Terán, A. Tirado-Guízar, R. M. Félix-Navarro, G. Alonso-Núñez and G. Pina-Luis, Anal. Methods, 2021, 13, 2495
DOI: 10.1039/D1AY00302J

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