Jump to main content
Jump to site search

Issue 16, 2021
Previous Article Next Article

Monoclonal antibody-based icELISA for the screening of diazinon in vegetable samples

Author affiliations

Abstract

Diazinon (DAZ) is an organophosphorus pesticide (OP), which is commonly used to prevent and control harmful pests that endanger agricultural products. In this study, we developed a novel heterology coating strategy for the immunoassay of DAZ. The DAZ coating hapten can be directly conjugated to the carrier protein without requiring a spacer arm. This proposed hapten coating strategy is time-saving and significantly improves the sensitivity of the immunoassay due to the lack of a spacer arm. The as-synthesized coating antigen was used to screen the monoclonal antibody (mAb). Finally, the developed indirect competitive enzyme-linked immunoassay (icELISA) showed IC50 and limit of detection (LOD) values of 0.58 ng mL−1 and 8 pg mL−1, respectively. This method exhibited negligible cross-reactivity towards other analogues, and the recoveries of samples (cucumber, cabbage, and lettuce) ranged from 92.6% to 125.4%, with coefficients of variance (CV) below 12%. Good correlation between icELISA and high-performance liquid chromatography mass spectrometry (HPLC-MS/MS) was obtained. The proposed icELISA was an ideal tool for monitoring DAZ residues in food samples.

Graphical abstract: Monoclonal antibody-based icELISA for the screening of diazinon in vegetable samples

Back to tab navigation

Supplementary files

Article information


Submitted
19 Feb 2021
Accepted
15 Mar 2021
First published
23 Mar 2021

Anal. Methods, 2021,13, 1911-1918
Article type
Paper

Monoclonal antibody-based icELISA for the screening of diazinon in vegetable samples

H. Wu, B. Wang, Y. Wang, Z. Xiao, L. Luo, Z. Chen, Y. Shen and Z. Xu, Anal. Methods, 2021, 13, 1911
DOI: 10.1039/D1AY00291K

Social activity

Search articles by author

Spotlight

Advertisements