Jump to main content
Jump to site search

Issue 18, 2021
Previous Article Next Article

Investigating the effect of 6-mercaptohexanol on the performance of a biosensor based on nanosurface energy transfer between gold nanoparticles and quantum dots

Author affiliations

Abstract

Nanosurface energy transfer (NSET)-based sensors have been widely developed using various pairs of nanomaterials including gold nanoparticles (AuNPs) and quantum dots (QDs). However, a low signal to background ratio is one of the most important problems that researchers are continually trying to solve. Herein, we present a 6-mercaptohexanol (MCH) modified MCH/DNA-Au-QD sensor for the detection of nucleic acids and MUC1. Interestingly, an unexpected effect of MCH was found in enhancing the fluorescence recovery ratio, therefore yielding a higher signal to background ratio. Through further investigation, we perceive the enhancement as a result of lowering of the NSET efficiency between free DNA-AuNPs and free DNA-QDs, which arises from the stretching of adsorbed DNA on the surface of AuNPs. The employment of MCH endowed the sensor with a wider linear range from 5 nM to 120 nM and a relatively lower LOD of 1.19 nM in nucleic acid detection, outperforming the original DNA-Au-QD sensor. Furthermore, the application of the sensor can be further extended to MUC1 detection. This study offers a better understanding of the NSET process between QDs and AuNPs and also initiates a new approach for the performance optimization of analogous NSET-based sensors.

Graphical abstract: Investigating the effect of 6-mercaptohexanol on the performance of a biosensor based on nanosurface energy transfer between gold nanoparticles and quantum dots

Back to tab navigation

Supplementary files

Article information


Submitted
04 Feb 2021
Accepted
25 Mar 2021
First published
25 Mar 2021

Anal. Methods, 2021,13, 2092-2098
Article type
Paper

Investigating the effect of 6-mercaptohexanol on the performance of a biosensor based on nanosurface energy transfer between gold nanoparticles and quantum dots

Q. Lai, Y. Liu, L. Ge, Y. Yang, X. Ji and Z. He, Anal. Methods, 2021, 13, 2092
DOI: 10.1039/D1AY00209K

Social activity

Search articles by author

Spotlight

Advertisements