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Issue 15, 2021
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A fluorescent aptasensor based on berberine for ultrasensitive detection of bisphenol A in tap water

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Abstract

The residues of bisphenol A (BPA) in food packaging and water systems have a potential impact on human health; therefore, its analysis and detection have drawn scientists' attention. In this work, based on the change in fluorescence intensity resulting from the conformational switch of a berberine/BPA-aptamer system in the presence and absence of BPA, an ultra-sensitive fluorescence aptasensing system is proposed, in which BPA-aptamer is employed as the identification unit and berberine as the fluorescent probe. Various factors affecting the detection of BPA, including the concentration of the fluorescent probe, BPA-aptamer, BPA, pH, system stability time and other experimental conditions, were investigated in detail. Under the optimal experimental conditions, the fluorescence intensity of the sensing system of berberine/BPA-aptamer exhibited a good linear correlation with the BPA concentration in the range of 0–1300 μM with a LOD of 32 nM. The proposed fluorescent sensing system also exhibited excellent recoveries of 92.4–102.3% in tap water samples and showed good application prospects for the analysis and detection of BPA.

Graphical abstract: A fluorescent aptasensor based on berberine for ultrasensitive detection of bisphenol A in tap water

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Supplementary files

Article information


Submitted
01 Feb 2021
Accepted
12 Mar 2021
First published
12 Mar 2021

Anal. Methods, 2021,13, 1816-1822
Article type
Paper

A fluorescent aptasensor based on berberine for ultrasensitive detection of bisphenol A in tap water

Y. Wei, Y. Zhou, Y. Wei, C. Dong and L. Wang, Anal. Methods, 2021, 13, 1816
DOI: 10.1039/D1AY00180A

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