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Issue 15, 2021
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An optic-fiber graphene field effect transistor biosensor for the detection of single-stranded DNA

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Abstract

Herein, a graphene field effect transistor (GFET) was constructed on an optic fiber end face to develop an integrated optical/electrical double read-out biosensor, which was used to detect target single-stranded DNA (tDNA). Two isolated Au electrodes were, respectively, prepared as the drain and source at the ends of an optic fiber and coated with a graphene film to construct a field effect transistor (FET). Probe aptamers modified with fluorophore 6′-carboxy-fluorescein (6′-FAM) were immobilized on the graphene for specific capture of tDNA. Graphene oxide (GO) was introduced to quench 6′-FAM and construct a fluorescence biosensor. Thus, a dual GFET and fluorescence biosensor was integrated on the end-face of an optic fiber. Following synchronous detection by fluorescence and FET methods, results showed satisfactory sensitivity for DNA detection. Compared with conventional biosensors using a single sensing technology, these dual sensing integrated biosensors significantly improved the reliability and accuracy of DNA detection. Furthermore, this proposed technique provides both a new biosensor for single-stranded DNA detection and a strategy for designing multi-sensing integrated biosensors.

Graphical abstract: An optic-fiber graphene field effect transistor biosensor for the detection of single-stranded DNA

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Article information


Submitted
18 Jan 2021
Accepted
09 Mar 2021
First published
09 Mar 2021

Anal. Methods, 2021,13, 1839-1846
Article type
Paper

An optic-fiber graphene field effect transistor biosensor for the detection of single-stranded DNA

Y. Zhang, Y. Ding, C. Li, H. Xu, C. Liu, J. Wang, Y. Ma, J. Ren, Y. Zhao and W. Yue, Anal. Methods, 2021, 13, 1839
DOI: 10.1039/D1AY00101A

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