Issue 1, 2021

A multi-location peak parking approach for calculation of second dimensional retention indices for improved volatile compound identification with cryogen-free comprehensive heart-cut two-dimensional gas chromatography

Abstract

Comprehensive heart-cut multidimensional gas chromatography (CH/C MDGC) without a cryogenic trapping device was developed with an established approach for calculation of first and second dimensional retention indices (1I and 2I) for improved compound identification. A first dimensional (1D) DB-1MS column (60 m) and a second dimensional (2D) DB-WAX column (60 m) were applied with a Deans switch (DS) using a constant H/C window of 0.2 min and a periodic multiple heartcut strategy comprising 225H/C throughout the CH/C. 1I was calculated based on comparison of the middle of the heartcut time with the alkane retention times on the 1D column. A multi-location peak parking approach using sixteen sets of automated injections of alkane references was also established with the least square curve fitting method for construction of the alkane isovolatility curves which were applied for 2I calculation. The untargeted compound analysis of a perfume sample was then performed according to comparison with the libraries of mass spectra, 1I and 2I. The CH/C MDGC system with a 25 h analysis time showed a peak capacity (nc) of 9198 and 128 separated peaks with 71 compounds successfully identified according to MS, 1I and 2I library match under the established error approximation criteria. Furthermore, relationship between the analysis time and number of separated peaks was proposed based on the set of 84 identifiable compounds. With the compensation of lower separation performance and greater I errors, the analysis time could be reduced by applying a 2.5 min H/C window with a total analysis time of 2 h and nc of 1134.

Graphical abstract: A multi-location peak parking approach for calculation of second dimensional retention indices for improved volatile compound identification with cryogen-free comprehensive heart-cut two-dimensional gas chromatography

Supplementary files

Article information

Article type
Paper
Submitted
23 Oct 2020
Accepted
24 Nov 2020
First published
15 Dec 2020

Anal. Methods, 2021,13, 124-132

A multi-location peak parking approach for calculation of second dimensional retention indices for improved volatile compound identification with cryogen-free comprehensive heart-cut two-dimensional gas chromatography

P. Janta, D. Pinyo, Y. Yodta, P. Vasasiri, M. Weidenbach, M. Pursch, X. (. Yang and C. Kulsing, Anal. Methods, 2021, 13, 124 DOI: 10.1039/D0AY01976C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements