Issue 20, 2021

Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe

Abstract

We successfully developed a fluorescent probe that can quickly convert full-length antibodies to Quenchbodies, which represent a type of fluorescent immunosensor with high binding affinity and specificity depending on the reaction of antigens and antibodies. An anti-testosterone IgG was successfully converted to an immunosensor that detects testosterone with a limit of detection (LOD) of 0.76 nM and concentration for 50% of maximal effect (EC50) of 61.5 nM. Another IgG-based immunosensor detected ractopamine with an LOD of 15.5 pM and EC50 of 48.6 nM.

Graphical abstract: Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe

Supplementary files

Article information

Article type
Communication
Submitted
02 Jul 2021
Accepted
27 Aug 2021
First published
03 Sep 2021

Analyst, 2021,146, 6114-6118

Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe

G. Zhao, H. Li, B. Li, M. Li, W. Xu, L. Chen, B. Wang and J. Dong, Analyst, 2021, 146, 6114 DOI: 10.1039/D1AN01171E

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