Issue 15, 2021

Target -responsive host–guest binding-driven dual-sensing readout for enhanced electrochemical chiral analysis

Abstract

Achieving efficient chiral discrimination by a convenient method remains a challenge in pharmaceutical and biotechnology industries. Our aim in this paper was to develop a dual-signaling enantioselective sensing strategy based on the competitive binding assay. A combination of β-cyclodextrin (β-CD) and methylene blue (MB) was used as an enantioselective discrimination probe to develop a straightforward electrochemical chiral sensor using the drug naproxen (R-and S-NaX) as the representative enantiomers. The principle relied on the difference between two enantiomers in the ability to replace a pre-binding redox probe, which in turn resulted in different dual signals for the two enantiomers. The applicability of the optimized procedure was demonstrated by the analysis of NaX enantiomers in the range of 0.4–6.0 μM. Featuring both signal-on and signal-off elements, the electrode presented significantly enhanced electrochemical activity with a low limit of detection (LOD) of 0.07 μM. We expect that our work will inspire interesting engineering strategies for developing novel enantioselective electrochemical sensors.

Graphical abstract: Target -responsive host–guest binding-driven dual-sensing readout for enhanced electrochemical chiral analysis

Article information

Article type
Paper
Submitted
05 May 2021
Accepted
21 Jun 2021
First published
25 Jun 2021

Analyst, 2021,146, 4865-4872

Target -responsive host–guest binding-driven dual-sensing readout for enhanced electrochemical chiral analysis

S. Ebrahimi, A. Afkhami and T. Madrakian, Analyst, 2021, 146, 4865 DOI: 10.1039/D1AN00795E

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