A facile colorimetric aptasensor for low-cost chlorpyrifos detection utilizing gold nanoparticle aggregation induced by polyethyleneimine

Abstract

A colorimetric aptasensor for chlorpyrifos detection utilizing the localized surface plasmon resonance (LSPR) of gold nanoparticle (AuNP) aggregates coupling with a specific aptamer and cationic polyethyleneimine (PEI) has been developed. The measurement principle is based on a remarkable characteristic of AuNPs that can change their colors under the aggregation and dispersion conditions, which enables a sensitive colorimetric detection. In the absence of chlorpyrifos, negatively charged phosphate backbones of the aptamer potentially interact with the cationic PEI, resulting in the red color appearance of the dispersed AuNPs, whereas, in the presence of chlorpyrifos, the aptamer binds explicitly to chlorpyrifos, consequently releasing cationic PEI. Uninteracted PEI induces AuNP aggregation, causing a color change from red to blue that can be observed through the naked eye. Under the optimized conditions, 6 nM PEI, 10 nM aptamer, and a pH buffer of 7.5, the colorimetric aptasensor gives a linear response in the range of 20–300 ng mL⁻¹ with a low detection limit of 7.4 ng mL⁻¹. The developed method has been successfully applied to complex sample analysis. The accuracy and precision of chlorpyrifos quantification in spiked samples, including tap water, pomelo, and longan samples, are in the acceptable criteria of method validation, indicating that the developed aptasensor can be utilized as an alternative analytical tool for chlorpyrifos determination in complex samples. This aptasensor provides advantages such as a simple procedure, low cost, short analysis time, and involving uncomplicated instruments. Moreover, it offers high sensitivity, selectivity, and stability.