Jump to main content
Jump to site search

Issue 7, 2021
Previous Article Next Article

Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode

Author affiliations

Abstract

Guanine (G) oxidation products, such as 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 8-oxo-guanine (8-OXOG), have been widely studied as promising biomarkers for DNA oxidative damage. In this work, we develop a new method to detect G oxidative products released from live cells after chromium (VI) ion or hydrogen peroxide treatments by using a glass nanopipette-based flexible gold nanoelectrode (fGNE). Specific response to G oxidative products with high sensitivity can be detected from the fGNE tip through integrated electrochemical measurements and surface-enhanced Raman spectroscopy. The fGNE apex can be positioned very close to the cell membrane noninvasively because of its high flexibility and nanoscale tip size. With the assistance of the electrophoretic force, the fGNEs can effectively collect and detect the G-derived DNA damage products released from individual cells in the cell culture medium with high sensitivity.

Graphical abstract: Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode

Back to tab navigation

Supplementary files

Article information


Submitted
03 Feb 2021
Accepted
09 Feb 2021
First published
11 Feb 2021

Analyst, 2021,146, 2321-2329
Article type
Paper

Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode

J. Zhou, D. Yang, G. Liu, S. Li, W. Feng, G. Yang, J. He and Y. Shan, Analyst, 2021, 146, 2321
DOI: 10.1039/D1AN00220A

Social activity

Search articles by author

Spotlight

Advertisements