Jump to main content
Jump to site search

Issue 8, 2021
Previous Article Next Article

Observation of inflammation-induced mitophagy during stroke by a mitochondria-targeting two-photon ratiometric probe

Author affiliations

Abstract

This study reports the development of a new, pH-sensitive, mitochondria-targeting two-photon ratiometric probe (Mito-BNO) for real-time tracking of mitophagy, a process that can be accelerated in brain tissue during stroke. Mito-BNO shows excellent capability for mitochondrial localisation (Pearson's correlation coefficient, r = 0.91), and can also effectively distinguish mitochondria from other subcellular organelles such as lysosomes and the endoplasmic reticulum (r = 0.40 and r = 0.33, respectively). Meanwhile, a rewarding pKa value (5.23 ± 0.03) and the pH reversibility suggest that Mito-BNO can track mitophagy in real time via confocal imaging. Most importantly, the relationship between mitophagy and neuroinflammation during stroke has been successfully demonstrated by evaluating the fluorescence of PC12 cells stained with Mito-BNO during an oxygen–glucose deprivation/reperfusion (OGD/R) process with and without anti-inflammatory treatment. The results indicate that the occurrence of mitophagy during stroke is caused by oxidative stress induced by neuroinflammation. This study will help further understanding stroke pathogenesis, can provide potential new targets for early diagnosis and treatment, and can also help to develop therapeutic drugs for stroke.

Graphical abstract: Observation of inflammation-induced mitophagy during stroke by a mitochondria-targeting two-photon ratiometric probe

Back to tab navigation

Supplementary files

Article information


Submitted
02 Feb 2021
Accepted
09 Feb 2021
First published
11 Feb 2021

Analyst, 2021,146, 2632-2637
Article type
Paper

Observation of inflammation-induced mitophagy during stroke by a mitochondria-targeting two-photon ratiometric probe

F. Cheng, T. Qiang, L. Ren, T. Liang, X. Gao, B. Wang and W. Hu, Analyst, 2021, 146, 2632
DOI: 10.1039/D1AN00208B

Social activity

Search articles by author

Spotlight

Advertisements