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Issue 7, 2021
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Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

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Abstract

Ten-eleven translocation (TET) dioxygenase is of great significance in cytosine demethylation and in the control of cell differentiation and transformation. Herein, a fluorescence method has been developed for the highly sensitive detection of TET1, a member of the TET dioxygenase family. Based on the ThT-induced specific conformation of the G-quadruplex structure, the fluorescence signal decreased linearly with increasing TET1 concentration. The method shows potential clinical applications in the screening of TET protein inhibitors for anticancer drug discovery.

Graphical abstract: Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

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Supplementary files

Article information


Submitted
18 Jan 2021
Accepted
04 Feb 2021
First published
08 Feb 2021

Analyst, 2021,146, 2126-2130
Article type
Communication

Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

X. Chen, Y. Cheng, Y. Wang, J. Tang, F. Wang and Z. Chen, Analyst, 2021, 146, 2126
DOI: 10.1039/D1AN00109D

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