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Issue 7, 2021
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Solubility of folic acid and protonation of folate in NaCl at different concentrations, even in physiological solution

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Abstract

The solubility of folic acid was determined at 25 °C in 1.00 mol dm−3 and in 0.15 mol dm−3 NaCl (physiological solution) spectrophotometrically by measuring the absorbance of saturated solution at different hydrogen ion concentrations. Five protonation constants of folate were determined both from the dependence of the solubility on the hydrogen ion concentration as well as from potentiometric titrations carried out in the presence of solid folic acid and in alkaline solution, in which folate is relatively soluble. Corresponding to the protonation constants, nuclear magnetic resonance and florescence spectra were also obtained at different hydrogen ion concentrations to determine the protonation positions in acid, neutral and alkaline solutions. An approach through circular dichroism was also applied to study the eventual polymerization of folate in alkaline solution.

Graphical abstract: Solubility of folic acid and protonation of folate in NaCl at different concentrations, even in physiological solution

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Article information


Submitted
05 Jan 2021
Accepted
08 Feb 2021
First published
11 Feb 2021

Analyst, 2021,146, 2339-2347
Article type
Paper

Solubility of folic acid and protonation of folate in NaCl at different concentrations, even in physiological solution

E. Bottari, A. D'Ambrosio, G. De Tommaso, M. R. Festa, M. Iuliano and M. Meschino, Analyst, 2021, 146, 2339
DOI: 10.1039/D1AN00013F

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