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Issue 5, 2021
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Nanosurface energy transfer indicating Exo III-propelled stochastic 3D DNA walkers for HIV DNA detection

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Abstract

DNA-based nanomachines have aroused tremendous interest because of their potential applications in bioimaging, biocomputing, and diagnostic treatment. Herein, we constructed a novel exonuclease III-propelled and signal-amplified stochastic DNA walker that autonomously walked on a spherical particle-based 3D track through a burnt-bridge mechanism, during which nanosurface energy transfer (NSET) occurred between the fluorescent dye modified on hairpin DNA and the surface of gold nanoparticles (AuNPs). As a proof of concept, this stochastic DNA walker achieves prominent detection performance of HIV DNA in the range of 0.05–1.2 nM with a detection limit of 12.7 pM and satisfactory recovery in blood serum, showing high promise in biosensing applications with complicated media.

Graphical abstract: Nanosurface energy transfer indicating Exo III-propelled stochastic 3D DNA walkers for HIV DNA detection

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Article information


Submitted
27 Nov 2020
Accepted
03 Feb 2021
First published
12 Feb 2021

Analyst, 2021,146, 1675-1681
Article type
Paper

Nanosurface energy transfer indicating Exo III-propelled stochastic 3D DNA walkers for HIV DNA detection

N. Wang, Y. J. Jiang, X. Zhang, H. R. Lin, F. Cheng, Q. Li, C. M. Li and C. Z. Huang, Analyst, 2021, 146, 1675
DOI: 10.1039/D0AN02289F

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