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Issue 8, 2021
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A novel microfluidic system for the sensitive and cost-effective detection of okadaic acid in mussels

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Abstract

Okadaic acid (OA) is produced by marine dinoflagellates and it can be easily accumulated in shellfish, causing intoxications when consumed by humans. Consequently, there is a need for sensitive, reliable and cost-effective methods to detect OA in real samples. In this work, we developed a novel and affordable microfluidic system to detect OA based on the protein phosphatase 1 inhibition colorimetric assay. This enzyme was immobilized in a microfluidic chamber by physisorption in an alumina sol–gel. The results show good enzyme stability over time when maintained at 4 °C. The developed system was sensitive for OA standard solutions, presenting a limit of detection (LOD) of 11.6 nM over a large linear range (43.4 to 3095.8 nM). Our method revealed an LOD as low as 0.2 μg kg−1 and a linear range between 1.47 and 506 μg kg−1 for extracted mussel matrix, detecting OA concentrations in contaminated mussels much lower than the regulated limit (160 μg kg−1). The enzyme stability and reusability along with the simplicity and low cost associated with microfluidics systems make this method very interesting from a commercial point of view.

Graphical abstract: A novel microfluidic system for the sensitive and cost-effective detection of okadaic acid in mussels

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Article information


Submitted
22 Oct 2020
Accepted
07 Feb 2021
First published
09 Feb 2021

Analyst, 2021,146, 2638-2645
Article type
Paper

A novel microfluidic system for the sensitive and cost-effective detection of okadaic acid in mussels

A. Castanheira, M. B. dos Santos, L. Rodriguez-Lorenzo, R. Queirós and B. Espiña, Analyst, 2021, 146, 2638
DOI: 10.1039/D0AN02092C

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