Issue 3, 2021

One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution

Abstract

Here we establish a one-pot reaction to directly convert the DNA base 5-hydroxymethylcytosine (5hmC) to an intramolecular cyclization nucleobase, which loses both protons of the exocyclic N4-amino group and thus is recognized as thymine (T) by DNA polymerase. Based on this 5hmC-specific reaction, a prospective bisulfite-free strategy for 5hmC sequencing is proposed. This is also the first example to show modified DNA labeling in non-water solvent-dominant media for DNA sequencing.

Graphical abstract: One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution

Supplementary files

Article information

Article type
Communication
Submitted
07 Oct 2020
Accepted
09 Dec 2020
First published
10 Dec 2020

Analyst, 2021,146, 820-824

One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution

Y. Ma, N. Zhang, S. Chen, J. Sun, Y. Liu, X. Li and H. Wang, Analyst, 2021, 146, 820 DOI: 10.1039/D0AN01981J

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