Issue 7, 2021

Highly sensitive and ratiometric luminescence sensing of heparin through templated cyanostilbene assemblies

Abstract

The assembly of organic dyes on bio-molecular templates is an attractive strategy for the creation of bio-materials with intriguing optical properties. This principle is exploited here for the detection of polyanion heparin, a known anticoagulant, by employing di-cationic cyanostilbene derivatives with inherent aggregation induced emission (AIE) features. The cyanostilbene derivatives exhibited weak cyan-blue monomeric emissions in solutions but upon electrostatic co-assembly with heparin, formed highly luminescent clusters on the polyanion surface. The cyanostilbene chromophores in the clusters exhibited greenish-yellow excimer emissions with remarkably longer life-times (up to 70-fold) and higher quantum yields (up to 85-fold) compared to their aqueous solutions. This led to heparin detection in aqueous buffer in low nanomolar concentrations. Additionally, and more importantly, a ratiometric detection of heparin was achieved in highly competitive media such as 50% human serum and 60% human plasma in medically relevant concentrations.

Graphical abstract: Highly sensitive and ratiometric luminescence sensing of heparin through templated cyanostilbene assemblies

Supplementary files

Article information

Article type
Paper
Submitted
08 Sep 2020
Accepted
04 Feb 2021
First published
10 Feb 2021

Analyst, 2021,146, 2194-2202

Highly sensitive and ratiometric luminescence sensing of heparin through templated cyanostilbene assemblies

S. K. Bhaumik and S. Banerjee, Analyst, 2021, 146, 2194 DOI: 10.1039/D0AN01808B

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