Issue 1, 2021

The preparation of bifunctional hybrid nano-flowers and their application in the enzyme-linked immunosorbent assay for Helicobacter pylori detection

Abstract

As the infection by Helicobacter pylori (H. pylori, HP) remains for a lifetime and may induce diseases such as gastric cancer, it is vital to detect and diagnose it. A new non-invasive indirect enzyme-linked immunosorbent assay (iELISA) method based on nano-flowers (NFs) is very advantageous for the sensitive detection of HP. Furthermore, the established iELISA method based on the organic–inorganic bifunctional hybrid nano-flowers including rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab–HRP@Cu2+ NFs) showed linearity with HP at a concentration of 0–105 CFU mL−1 (R2 = 0.9997). Moreover, the limit of detection (LOD) reached 50 CFU mL−1, and not only was the detection sensitivity 20 times higher than that based on rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab–HRP) but also the stability of R-HP-Ab–HRP in NFs was improved. In addition, the OD450 nm value was still linearly related to the concentration of HP at a range of 0–105 CFU mL−1 (R2 = 0.9952) with a LOD of 50 CFU mL−1 in an artificial saliva system. This study provided a sensitive, low-cost and convenient method for the non-invasive detection of HP.

Graphical abstract: The preparation of bifunctional hybrid nano-flowers and their application in the enzyme-linked immunosorbent assay for Helicobacter pylori detection

Supplementary files

Article information

Article type
Paper
Submitted
02 Aug 2020
Accepted
06 Oct 2020
First published
07 Nov 2020

Analyst, 2021,146, 338-347

The preparation of bifunctional hybrid nano-flowers and their application in the enzyme-linked immunosorbent assay for Helicobacter pylori detection

T. Wang, X. Li, L. Chen, Y. Zhang, Y. Zheng, L. Yu, Z. Ye, H. Wang, X. Cui and S. Zhao, Analyst, 2021, 146, 338 DOI: 10.1039/D0AN01533D

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