SYPRO Orange – a new gold standard amyloid probe

Abstract

SYPRO Orange, a zwitterionic merocyanine dye, can strongly interact with amyloid fibrils and shows enormous (∼1200 times) increase in its emission intensity. The sensitivity of the new probe is several times higher than that of the gold standard amyloid probe thioflavin T. Unlike thioflavin-T, the new probe has spectral properties suitable for in vivo imaging with detection sensitivity in the picomolar range.