Issue 45, 2020

A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

Abstract

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity. We demonstrated that the dephosphorylation of the water-soluble, phosphate-functionalized, fluorophore monomer P-TPE-TG, induced by an enzymatic reaction of ALP, leads to micelle formation in aqueous solution because its water-soluble functionality is reduced. The dephosphorylation-induced micellization of P-TPE-TG exhibited a ratiometric sensing response for various ALP concentrations (10–200 mU mL−1) and provided a suitable sensing platform for naked eye detection with increased fluorescence quantum yield (Φ = 3.2%), even compared to a typical TPE-based sensor (Φ = 1.0%), where ALP can be sensed with a detection limit of 0.034 mU mL−1. In addition, P-TPE-TG displayed excellent sensing performance at concentrations from 0 to 50 mU mL−1 in diluted human serum (10%), which offers the capability to exploit ratiometric responses for bioactive substances under practical conditions.

Graphical abstract: A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

Supplementary files

Article information

Article type
Paper
Submitted
21 Apr 2020
Accepted
09 Jul 2020
First published
20 Jul 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 26888-26894

A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

J. Lee, S. Kim, T. H. Kim and S. H. Lee, RSC Adv., 2020, 10, 26888 DOI: 10.1039/D0RA03584J

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