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Issue 4, 2020, Issue in Progress
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Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

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Abstract

A literature survey revealed no suitable “reversed phase-high performance thin layer chromatography (RP-HPTLC)” method for the analysis of rivaroxaban in nanoparticle (NP) formulations. Therefore, a novel rapid, simple, economical and environment friendly RP-HPTLC method has been established for the quantification of rivaroxaban in NP formulations and commercial tablets. RP-HPTLC analysis of rivaroxaban was performed using “RP-18 silica gel 60 F254S HPTLC plates”. The binary mixture of green solvents ethanol : water (7 : 3 v/v) was utilized as the mobile phase. The quantification of rivaroxaban was carried out in densitometric mode at λmax = 253 nm. Rivaroxaban peaks from NP formulations was confirmed by comparing its single spot at Rf = 0.71 ± 0.02 with that of the standard. The proposed RP-HPTLC method was found to be linear in the range of 50–600 ng per band with R2 = 0.9994. The equation for linear regression analysis was obtained as Y = 13.28x + 1189.4. The proposed RP-HPTLC technique was validated for “precision, accuracy, robustness and sensitivity”. The accuracy of the method was obtained as 97.97–99.67%. The % RSD in repeatability and intermediate precision was recorded as 0.46–0.64 and 0.48–0.86%, respectively. The LOD and LOQ for rivaroxaban were obtained as 18.45 and 55.35 ng per spot, respectively. The % content of rivaroxaban in marketed tablets and NPs was recorded as 99.20 and 98.08%, respectively. The proposed RP-HPTLC technique could be successfully applied for the pharmaceutical assay of rivaroxaban in NPs, marketed tablets and related formulations.

Graphical abstract: Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

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Article information


Submitted
26 Sep 2019
Accepted
03 Jan 2020
First published
10 Jan 2020

This article is Open Access

RSC Adv., 2020,10, 2133-2140
Article type
Paper

Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

P. Alam, E. Ezzeldin, M. Iqbal, Md. K. Anwer, G. A. E. Mostafa, M. H. Alqarni, A. I. Foudah and F. Shakeel, RSC Adv., 2020, 10, 2133
DOI: 10.1039/C9RA07825H

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