Issue 15, 2020

Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

Abstract

A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.

Graphical abstract: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

Associated articles

Supplementary files

Article information

Article type
Communication
Submitted
11 Mar 2020
Accepted
19 Mar 2020
First published
19 Mar 2020

Org. Biomol. Chem., 2020,18, 2813-2816

Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

Y. Morishita, T. Sonohara, T. Taniguchi, K. Adachi, M. Fujita and T. Asai, Org. Biomol. Chem., 2020, 18, 2813 DOI: 10.1039/D0OB00519C

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