Issue 45, 2020

Palladium(ii) complexes bearing 1-iminothiolate-3,5-dimethylpyrazoles: synthesis, cytotoxicity, DNA binding and enzymatic inhibition studies

Abstract

Four palladium(II) compounds of general formulae [PdCl(Ln)(PPh3)] {L1 = 3,5-dimethylpyrazole-1-iminothiolate (1); L2 = 3,5-dimethyl-pyrazole-N-methyl-1-iminothiolate (2); L3 = 3,5-dimethylpyrazole-N-ethyl-1-iminothiolate (3); L4 = 3,5-dimethylpyrazole-N-phenyl-1-iminothiolate (4); and PPh3 = triphenylphosphine} have been synthesized. The novel synthesized compounds have been characterized by C, H and N elemental analysis, 1D (1H and 13C) and 2D (HSQC and HMBC) NMR, MS, FT-IR, and molar electrical conductivity measurements. The molecular structure of complex 3 has been solved by single-crystal X-ray crystallography. The stability of the complexes in solution was studied in a DMSO/D2O (7 : 3) solution after 48 h. The antiproliferative activity of all free ligands and the stable palladium complexes 24 was assayed using the human breast tumour cell line MCF-7, lung tumour cell line A549 and human fetal lung fibroblast cell line MRC-5. Complex 3 was more active than cisplatin against MCF-7 cells, whilst palladium compounds 2–4 exhibited no drug response towards A549 cells at concentrations <50 μM. The binding properties of compounds 2 and 3 to ct-DNA have been studied using circular dichroism and fluorescence spectroscopy. The topoisomerase IIα inhibition has been studied for complex 2 and 3. The ability of all complexes to inhibit the activity of cathepsin B and L has also been investigated in this work. Compound 4 inhibited more than 50% of the cathepsin B activity at a concentration of 10 μM. Docking simulations have been carried out to gain more information about the interaction of the complexes and cathepsin B.

Graphical abstract: Palladium(ii) complexes bearing 1-iminothiolate-3,5-dimethylpyrazoles: synthesis, cytotoxicity, DNA binding and enzymatic inhibition studies

Supplementary files

Article information

Article type
Paper
Submitted
03 Jun 2020
Accepted
17 Oct 2020
First published
21 Oct 2020

New J. Chem., 2020,44, 19891-19901

Palladium(II) complexes bearing 1-iminothiolate-3,5-dimethylpyrazoles: synthesis, cytotoxicity, DNA binding and enzymatic inhibition studies

T. R. de Moura, R. D. Zanetti, D. E. S. Silva, R. L. de Farias, A. E. Mauro, J. C. M. Pereira, A. A. de Souza, F. da Silva Siqueira, W. A. de Souza Júdice, M. A. Lima, F. V. Rocha, V. M. Deflon and A. Vieira de Godoy Netto, New J. Chem., 2020, 44, 19891 DOI: 10.1039/D0NJ02825H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements