Effect of tamarind seed polysaccharide on thermogelation property and drug release profile of poloxamer 407-based ophthalmic formulation

Abstract

Herein, the potential impact of tamarind seed polysaccharide (TSP) on the gelation nature and in vitro release of a particular drug, pilocarpine hydrochloride, from different poloxamer 407-based ophthalmic formulations were evaluated. The combination of TSP (0.5–1.5 wt%) and 18 wt% poloxamer (PM) solutions resulted in a significant improvement in gel elasticity under physiological conditions. The introduction of TSP to PM not only enhanced the viscosity of the gel, but also stimulated the gelation procedure. Due to the inclusion of TSP, the gel dissolution rate of the PM-based formulation was greatly reduced. The FTIR spectral analysis revealed the interaction between –OH groups of both polymers, resulting in a decline in the gelation temperature of the PM–TSP-based formulations. It was also detected in the cryo-SEM analysis that the pore size of the PM gel decreased and the depth of its pores increased with the inclusion of TSP. Furthermore, the in vitro release analysis indicated that the retention capacity of PM–TSP-based formulations was superior to that of the only PM-based formulation. The SEM study and the cell viability data demonstrated the biocompatibility and non-toxicity of TSP. Therefore, the designed formulation is fairly promising to be utilized as an in situ ophthalmic drug carrier.