A nanocomposite of NiFe2O4–PANI as a duo active electrocatalyst toward the sensitive colorimetric and electrochemical sensing of ascorbic acid

Abstract

A non-enzymatic ascorbic acid sensor using a nickel ferrite/PANI (NF–PANI) nanocomposite and based on colorimetric and electrochemical sensing methods was investigated in this study. The nanocomposite was prepared by an in situ polymerization and utilized as an electrocatalyst to sense ascorbic acid (AA) through the peroxidase mimic sensing of H₂O₂ in the presence of 3,5,3,5-tetramethylbenzidine (TMB) as a coloring agent. It was also utilized to detect AA present in real samples prepared from fruit extracts, commercial beverages, and vitamin-C tablets. The limit of detection (LoD) for AA sensing by the peroxidase mimic method was found to be 232 nM. The relative standard deviation (RSD) calculated for analysis of the real samples analysis ranged from 1.7–3.2%. Similarly, the electrochemical sensing of AA by NF–PANI was examined by cyclic voltammetric, chronoamperometric, and differential pulse voltammetric analyses. The LoD for the electrochemical method applied to AA sensing was 423 nM. The nanocomposite functioned as an effective electrocatalytic sensing agent in both methods to selectively detect AA due to the combined effect of NF and PANI. Thus, it was shown that the nanocomposites could be utilized for the laboratory-based detection of AA by various methods and could give rapid results.