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Issue 9, 2020
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Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis

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Abstract

Fenretinide is a synthetic retinoid pharmaceutical linked to ceramide build-up in vivo. Saposin D is an intralysosomal protein necessary for ceramide binding/degradation. We show, via electronic absorption spectroscopy, fluorescence spectroscopy, and ceramide hydrolysis assays, that fenretinide is bound by saposin D {Ka = (1.45 ± 0.49) × 105 M−1}, and affects ceramide solubilization/degradation.

Graphical abstract: Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis

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Supplementary files

Article information


Submitted
30 May 2020
Accepted
26 Jun 2020
First published
14 Jul 2020

RSC Med. Chem., 2020,11, 1048-1052
Article type
Research Article

Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis

B. T. Milliken, L. Melegari, G. L. Smith, K. Grohn, A. J. Wolfe, K. Moody, F. Bou-Abdallah and R. P. Doyle, RSC Med. Chem., 2020, 11, 1048
DOI: 10.1039/D0MD00182A

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