In vitro anti-diabetic assessment of guavanoic acid functionalized gold nanoparticles in regulating glucose transport using L6 rat skeletal muscle cells

Abstract

Guavanoic acid functionalized gold nanoparticles exhibit anti-diabetic potential by improving insulin dependent glucose uptake in L6 rat skeletal muscle cells. The mode of action of the gold nanoparticles was established from the glucose uptake assay in the presence and absence of genistein and wortmannin. The anti-diabetic efficacy of guavanoic acid functionalized gold nanoparticles was put forth by in vitro assays like for PTP 1B, α-amylase and α-glucosidase enzyme activities. Studies on cytotoxicity revealed 50% inhibition of cells at 265 ± 0.01 μg mL⁻¹. In the LDH enzyme release assay on differentiated L6 myoblasts treated with different concentrations (1–100 μg mL⁻¹) of guavanoic acid functionalized gold nanoparticles, a viability of 75% at 100 μg mL⁻¹ was observed.