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Anticancer potential of exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cell via apoptosis induction

Abstract

This study aimed at investigating the anticancer activity of the exopolysaccharide (EPS) isolated from Lactobacillus helveticus MB2-1. The crude EPS from L. helveticus MB2-1 (LHEPS) was fractionated into three fractions, namely LHEPS-1, LHEPS-2 and LHEPS-3. LHEPS-1 exhibited the most effective anti-proliferative activity, which was associated with a stronger inhibition rate and increased lactate dehydrogenase leakage of human colon cancer HT-29 cells. Flow cytometry analyses and colorimetric assay revealed that LHEPS-1 induced the cell cycle arrest by preventing G1 to S transmission and increased apoptosis rate. Furthermore, LHEPS-1 enhanced the production of intracellular reactive oxygen species (ROS) and the activity of caspase-8/9/3, increased the levels of pro-apoptotic (Bax) and mitochondrial cytochrome c, while decreased the anti-apoptotic (Bcl-2) level, indicating that LHEPS-1 might induce apoptosis of HT-29 cells through a ROS-dependent pathway and a mitochondria-dependent pathway. These findings suggest that LHEPS-1 may be developed as an effective food and/or drug for prevention and therapeutics of cancer, especially for human colon cancer.

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Article information


Submitted
23 May 2020
Accepted
15 Oct 2020
First published
16 Oct 2020

Food Funct., 2020, Accepted Manuscript
Article type
Paper

Anticancer potential of exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cell via apoptosis induction

L. Xiao, X. Ge, L. Yang, X. Chen, Q. Xu, X. Rui, X. Fan, L. Feng, Q. Zhang, M. Dong and W. Li, Food Funct., 2020, Accepted Manuscript , DOI: 10.1039/D0FO01345E

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