Issue 35, 2020

Systematic spectroscopic investigation of structural changes and corona formation of bovine serum albumin over magneto-fluorescent nanoparticles

Abstract

Magneto-fluorescent nanoparticles have attracted great attention because of their dual nature as multimodal imaging probes in various biomedical applications. Particulary, it is desirable to understand how these nanoparticles interact with human serum proteins before they are used in biological systems. In this work, the physiological interactions between magneto-fluorescent nanoparticles (MFNPs) and bovine serum albumin (BSA) were systematically investigated. The present observations identified that the collision of MFNPs and BSA caused fluorescence quenching of BSA. Steady state fluorescence, lifetime and anisotropy measurements in the presence of MFNPs supported dynamic quenching of the BSA emission. Furthermore, in the presence of MFNPs, the conformation changes in the BSA structure indicate merely the secondary structural changes. The findings demonstrated that random interactions and hydrophobic forces play a major role in the dynamic quenching. Further, subsequent coating of BSA over MFNPs result in protein corona formation, as is evident through spectroscopic measurements. In light of the present work, MFNPs could serve a purpose in magnetic separation and optical detection of serum proteins from blood plasma in real-time disease diagnosis.

Graphical abstract: Systematic spectroscopic investigation of structural changes and corona formation of bovine serum albumin over magneto-fluorescent nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
08 Jul 2020
Accepted
04 Aug 2020
First published
05 Aug 2020

Dalton Trans., 2020,49, 12380-12389

Systematic spectroscopic investigation of structural changes and corona formation of bovine serum albumin over magneto-fluorescent nanoparticles

A. Tiwari, P. Bhatia and J. K. Randhawa, Dalton Trans., 2020, 49, 12380 DOI: 10.1039/D0DT02414G

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