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Issue 21, 2020
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Genetic alphabet expansion technology by creating unnatural base pairs

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Abstract

Recent advancements in the creation of artificial extra base pairs (unnatural base pairs, UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet expansion technologies. UBPs that function as third base pairs in replication, transcription, and/or translation enable the site-specific incorporation of novel components into DNA, RNA, and proteins. Here, we describe the UBPs developed by three research teams and their application in PCR-based diagnostics, high-affinity DNA aptamer generation, site-specific labeling of RNAs, semi-synthetic organism creation, and unnatural-amino-acid-containing protein synthesis.

Graphical abstract: Genetic alphabet expansion technology by creating unnatural base pairs

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Submitted
28 Apr 2020
First published
05 Oct 2020

Chem. Soc. Rev., 2020,49, 7602-7626
Article type
Review Article

Genetic alphabet expansion technology by creating unnatural base pairs

M. Kimoto and I. Hirao, Chem. Soc. Rev., 2020, 49, 7602
DOI: 10.1039/D0CS00457J

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