Issue 39, 2020

Theoretical study on the catalytic mechanism of human deoxyhypusine hydroxylase

Abstract

Deoxyhypusine hydroxylase is a critical enzyme for hypusination of eukaryotic translation initiation factor 5A (eIF5A). Human deoxyhypusine hydroxylase (hDOHH) has a nonheme diiron active site that resembles both in structure and function of those found in methane and toluene monooxygenases, bacterial and mammalian ribonucleotide reductases, and stearoyl acyl carrier protein Δ9-desaturase from plants. However, the detailed catalytic mechanism of hDOHH is still unclear. In this work, extensive DFT calculations reveal that the catalytic mechanism of hDOHH consists of four consecutive steps: (1) peroxo isomerization triggered by substrate binding; (2) rate-determining O–O bond cleavage and formation of the [FeIV2(μ-O)2]4+ compound; (3) H atom abstraction from the substrate; and (4) OH rebound to the substrate. This work not only rationalizes the exceptional stability of the diiron(III)-peroxo complex in hDOHH, but also confirms that hDOHH uses a diamond shape [FeIV2(μ-O)2]4+ core to complete crucial H atom abstraction from the substrate. Our DFT calculations exclude the reaction pathway of hDOHH to use diiron(III)-peroxo species to directly react with the substrate.

Graphical abstract: Theoretical study on the catalytic mechanism of human deoxyhypusine hydroxylase

Supplementary files

Article information

Article type
Paper
Submitted
06 Jul 2020
Accepted
14 Sep 2020
First published
16 Sep 2020

Phys. Chem. Chem. Phys., 2020,22, 22736-22745

Theoretical study on the catalytic mechanism of human deoxyhypusine hydroxylase

J. Wang, Y. Ma, X. Wang, Y. Zhang, H. Tan, X. Li and G. Chen, Phys. Chem. Chem. Phys., 2020, 22, 22736 DOI: 10.1039/D0CP03598J

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