Jump to main content
Jump to site search

Issue 1, 2021

A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Author affiliations

Abstract

A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.

Graphical abstract: A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Supplementary files

Article information


Submitted
22 Oct 2020
Accepted
30 Nov 2020
First published
08 Dec 2020

This article is Open Access

Chem. Commun., 2021,57, 97-100
Article type
Communication

A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

M. R. Rios, G. Garoffolo, G. Rinaldi, A. Megia-Fernandez, S. Ferrari, C. T. Robb, A. G. Rossi, M. Pesce and M. Bradley, Chem. Commun., 2021, 57, 97 DOI: 10.1039/D0CC07028A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements