Chemical synthesis of ferredoxin with 4 selenocysteine residues using a segment condensation method

Abstract

Ferredoxin (Fd) is an electron carrier protein containing a [2Fe–2S] cluster. In this paper, we synthesized Se–Fd, in which four Cys residues coordinated to the cluster are substituted to selenocysteine. After the one-pot segment coupling by the thioester method, followed by deprotection and cluster loading, the desired Se–Fd was successfully obtained.