A bimolecular i-motif mediated FRET strategy for imaging protein homodimerization on a living tumor cell surface†
Abstract
A bimolecular i-motif mediated FRET strategy was developed based on the proximity-induced folding of two identical cytosine-rich DNA strands. This strategy affords a FRET signal that is highly matched to the dimerization event, and enabled accurate and dynamic in situ imaging of Met homodimerization on a living tumor cell surface.