Issue 68, 2020
From the journal:

Chemical Communications

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery†

Xuehe Lu,ab   Xiaohui Wu,bc   Tiantian Wu,bc   Lin Han,ab   Jianbing Liu*bc  and  Baoquan Ding ORCID logo *abc  

* Corresponding authors

a School of Materials Science and Engineering, Henan Institute of Advanced Technology, Zhengzhou University, Zhengzhou, China

b CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China
E-mail: liujb@nanoctr.cn, dingbq@nanoctr.cn

c University of Chinese Academy of Sciences, Beijing, China

Abstract

A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-L-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.

Graphical abstract: Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

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Article information

DOI
https://doi.org/10.1039/D0CC04356G
Article type
Communication
Submitted
23 Jun 2020
Accepted
18 Jul 2020
First published
21 Jul 2020

Chem. Commun., 2020,56, 9894-9897

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Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

X. Lu, X. Wu, T. Wu, L. Han, J. Liu and B. Ding, Chem. Commun., 2020, 56, 9894 DOI: 10.1039/D0CC04356G

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