An improved structure-switch aptamer-based fluorescent Pb2+ biosensor utilizing the binding induced quenching of AMT to G-quadruplex

Fenghua Geng; Dandan Wang; Li Feng; Guixin Li; Maotian Xu

doi:10.1039/D0CC03669B

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An improved structure-switch aptamer-based fluorescent Pb²⁺ biosensor utilizing the binding induced quenching of AMT to G-quadruplex†

Fenghua Geng,

‡^ab Dandan Wang,‡^c Li Feng,*^a Guixin Li^c and Maotian Xu

*^b

Author affiliations

* Corresponding authors

^a National Engineering Research Center of Coal Preparation and Purification, Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, School of Chemical Engineering and Technology, China University of Mining & Technology, Xuzhou, China
E-mail: 8422127@alu.fudan.edu.cn

^b Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, China
E-mail: xumaotian@sqnu.edu.cn

^c College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, China

Abstract

An improved aptamer-based fluorescent Pb²⁺ biosensor utilizing the binding induced quenching of AMT to G-quadruplex has been rationally designed with a LOD of 3.6 nM. The utility of the developed biosensor was demonstrated by the successful detection of Pb²⁺ in real complex clinical samples with satisfactory recovery and good reproducibility.

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Article information

DOI: https://doi.org/10.1039/D0CC03669B
Article type: Communication
Submitted: 23 May 2020
Accepted: 24 Jul 2020
First published: 25 Jul 2020

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Chem. Commun., 2020,56, 10517-10520

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An improved structure-switch aptamer-based fluorescent Pb²⁺ biosensor utilizing the binding induced quenching of AMT to G-quadruplex

F. Geng, D. Wang, L. Feng, G. Li and M. Xu, Chem. Commun., 2020, 56, 10517 DOI: 10.1039/D0CC03669B

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