H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions

Ammar Al-Shameri; Sébastien J.-P. Willot; Caroline E. Paul; Frank Hollmann; Lars Lauterbach

doi:10.1039/D0CC03229H

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H₂ as a fuel for flavin- and H₂O₂-dependent biocatalytic reactions†

Ammar Al-Shameri,

^a Sébastien J.-P. Willot,^b Caroline E. Paul,

*^b Frank Hollmann

*^b and Lars Lauterbach

*^a

Author affiliations

* Corresponding authors

^a Institute of Chemistry, Technische Universität Berlin, Strasse des 17, Juni 135, 10623 Berlin, Germany
E-mail: lars.lauterbach@tu-berlin.de

^b Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands
E-mail: f.hollmann@tudelft.nl, c.e.paul@tudelft.nl

Abstract

The soluble hydrogenase from Ralstonia eutropha provides an atom efficient regeneration system for reduced flavin cofactors using H₂ as an electron source. We demonstrated this system for highly selective ene-reductase-catalyzed C [double bond, length as m-dash] C-double bond reductions and monooxygenase-catalyzed epoxidation. Reactions were expanded to aerobic conditions to supply H₂O₂ for peroxygenase-catalyzed hydroxylations.

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Correction: H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions

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Article information

DOI: https://doi.org/10.1039/D0CC03229H
Article type: Communication
Submitted: 05 May 2020
Accepted: 14 Jul 2020
First published: 14 Jul 2020
This article is Open Access

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Chem. Commun., 2020,56, 9667-9670

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H₂ as a fuel for flavin- and H₂O₂-dependent biocatalytic reactions

A. Al-Shameri, S. J.-P. Willot, C. E. Paul, F. Hollmann and L. Lauterbach, Chem. Commun., 2020, 56, 9667 DOI: 10.1039/D0CC03229H

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