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Development of Bidirectional Isothermal Amplification Strategy for Sensitive Detection of Transcription Factors in Cancer Cells

Abstract

We develop a new strategy to sensitively detect transcription factors (TFs) based on the integration of bidirectional isothermal exponential amplification reaction (EXPAR) with endonuclease IV (Endo IV)-assisted cycle digestion of signal probes. This assay exhibits ultrahigh sensitivity with a detection limit of 1.29 × 10-14 M, and it can measure endogenous NF-κB p50 in HeLa cell extracts. Moreover, this strategy can be applied to screen TF inhibitors and detect other TFs by simply changing the TF-binding sequence.

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Article information


Submitted
30 Apr 2020
Accepted
30 Jun 2020
First published
30 Jun 2020

Chem. Commun., 2020, Accepted Manuscript
Article type
Communication

Development of Bidirectional Isothermal Amplification Strategy for Sensitive Detection of Transcription Factors in Cancer Cells

Y. Zhang, Q. Li, D. Xiang, K. Zhou, Q. Xu and C. Zhang, Chem. Commun., 2020, Accepted Manuscript , DOI: 10.1039/D0CC03134H

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