Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy

Lara Williams; Sonja Tischlik; Andreas Scherer; Jörg Wolfram Anselm Fischer; Malte Drescher

doi:10.1039/D0CC03101A

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy†

Lara Williams,

‡^a Sonja Tischlik,

‡^a Andreas Scherer,

^a Jörg Wolfram Anselm Fischer

^a and Malte Drescher

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany
E-mail: malte.drescher@uni-konstanz.de

Abstract

Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD) spectroscopy in proteins that lack an intrinsic photoexcitable center.

Download options Please wait...

Supplementary files

Supplementary information PDF (3631K)

Article information

DOI: https://doi.org/10.1039/D0CC03101A
Article type: Communication
Submitted: 29 Apr 2020
Accepted: 04 Aug 2020
First published: 07 Nov 2020
This article is Open Access

Download Citation

Chem. Commun., 2020,56, 14669-14672

Permissions

Request permissions

Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy

L. Williams, S. Tischlik, A. Scherer, J. W. A. Fischer and M. Drescher, Chem. Commun., 2020, 56, 14669 DOI: 10.1039/D0CC03101A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy

Social activity

Search articles by author

Spotlight

Advertisements